Citation:

jpy.12347

Name: Microsensor studies on Padina from a natural CO2 seep: implications of morphology on acclimation to low pH
Published: 2015
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Benthos; Bottles or small containers/Aquaria (<20 L); Chlorophyta; Chromista; CO2 vent; Coast and continental shelf; Field experiment; Macroalgae; Ochrophyta; Padina australis; Primary production/Photosynthesis; Single species; South Pacific; Temperate

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Abstract:

Low seawater pH can be harmful to many calcifying marine organisms, but the calcifying macroalgae Padina spp. flourish at natural submarine carbon dioxide seeps where seawater pH is low. We show that the microenvironment created by the rolled thallus margin of Padina australis facilitates supersaturation of CaCO3 and calcifi-cation via photosynthesis-induced elevated pH. Using microsensors to investigate oxygen and pH dynamics in the microenvironment of P. australis at a shallow CO2 seep, we found that, under saturating light, the pH inside the microenvironment (pHME) was higher than the external seawater (pHSW) at all pHSW levels investigated, and the difference (i.e., pHME-pHSW) increased with decreasing pHSW (0.9 units at pHSW 7.0). Gross photosynthesis (Pg) inside the microenvironment increased with decreasing pHSW, but algae from the control site reached a threshold at pH 6.5. Seep algae showed no pH threshold with respect to Pg within the pHSW range investigated. The external carbonic anhydrase (CA) inhibitor, acetazolamide, strongly inhibited Pg of P. australis at pHSW 8.2, but the effect was diminished under low pHSW (6.4-7.5), suggesting a greater dependence on membrane-bound CA for the dehydration of HCO3- ions during dissolved inorganic carbon uptake at the higher pHSW. In comparison, a calcifying green alga, Halimeda cuneata f. digitata, was not inhibited by AZ, suggesting efficient bicarbonate transport. The ability of P. australis to elevate pHME at the site of calcification and its strong dependence on CA may explain why it can thrive at low pHSW.

Keyword(s):

Benthos; Bottles or small containers/Aquaria (<20 L); Chlorophyta; Chromista; CO2 vent; Coast and continental shelf; Field experiment; Macroalgae; Ochrophyta; Padina australis; Primary production/Photosynthesis; Single species; South Pacific; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-05-05.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Hofmann, Laurie C		study
2	Species	Species		Hofmann, Laurie C
3	Registration number of species	Reg spec no		Hofmann, Laurie C
4	Uniform resource locator/link to reference	URL ref		Hofmann, Laurie C		WoRMS Aphia ID
5	Figure	Fig		Hofmann, Laurie C
6	Site	Site		Hofmann, Laurie C
7	Treatment	Treat		Hofmann, Laurie C
8	Identification	ID		Hofmann, Laurie C
9	Run Date/Time	Run Date/Time		Hofmann, Laurie C
10	Distance	Distance	µm	Hofmann, Laurie C		depth from inside microenvironment
11	Oxygen	O2	µmol/l	Hofmann, Laurie C
12	Oxygen, standard error	O2 std e	±	Hofmann, Laurie C
13	Distance	Distance	µm	Hofmann, Laurie C		depth from thallus surface
14	Oxygen	O2	µmol/l	Hofmann, Laurie C		corrected for drift
15	Location	Location		Hofmann, Laurie C
16	Identification	ID		Hofmann, Laurie C		individual
17	Replicate	Repl		Hofmann, Laurie C
18	Gross photosynthesis rate, oxygen	PG O2	nmol/cm³/s	Hofmann, Laurie C
19	Gross photosynthesis rate, oxygen	PG O2	nmol/cm³/s	Hofmann, Laurie C		average
20	Salinity	Sal		Hofmann, Laurie C
21	Temperature, water	Temp	°C	Hofmann, Laurie C
22	pH	pH		Hofmann, Laurie C	Potentiometric	free scale
23	Carbon, inorganic, dissolved	DIC	µmol/kg	Hofmann, Laurie C
24	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
26	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

40403 data points

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