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Liu, Fengjie; Gledhill, Martha; Tan, Qiaoguo; Zhu, Kechen; Zhang, Qiong; Salaün, Pascal; Tagliabue, Alessandro; Zhang, Yanjun; Weiss, Dominik J; Achterberg, Eric Pieter; Korchev, Yuri (2022): Seawater carbonate chemistry and the decrease of H+ concentration in the phycosphere and thickness of the pH boundary layer of marine diatoms Coscinodiscus wailesii. PANGAEA, https://doi.org/10.1594/PANGAEA.951332

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Abstract:
Surface ocean pH is declining due to anthropogenic atmospheric CO2 uptake with a global decline of ~0.3 possible by 2100. Extracellular pH influences a range of biological processes, including nutrient uptake, calcification and silicification. However, there are poor constraints on how pH levels in the extracellular microenvironment surrounding phytoplankton cells (the phycosphere) differ from bulk seawater. This adds uncertainty to biological impacts of environmental change. Furthermore, previous modelling work suggests that phycosphere pH of small cells is close to bulk seawater, and this has not been experimentally verified. Here we observe under 140 μmol photons/m**2/s the phycosphere pH of Chlamydomonas concordia (5 µm diameter), Emiliania huxleyi (5 µm), Coscinodiscus radiatus (50 µm) and C. wailesii (100 µm) are 0.11 ± 0.07, 0.20 ± 0.09, 0.41 ± 0.04 and 0.15 ± 0.20 (mean ± SD) higher than bulk seawater (pH 8.00), respectively. Thickness of the pH boundary layer of C. wailesii increases from 18 ± 4 to 122 ± 17 µm when bulk seawater pH decreases from 8.00 to 7.78. Phycosphere pH is regulated by photosynthesis and extracellular enzymatic transformation of bicarbonate, as well as being influenced by light intensity and seawater pH and buffering capacity. The pH change alters Fe speciation in the phycosphere, and hence Fe availability to phytoplankton is likely better predicted by the phycosphere, rather than bulk seawater. Overall, the precise quantification of chemical conditions in the phycosphere is crucial for assessing the sensitivity of marine phytoplankton to ongoing ocean acidification and Fe limitation in surface oceans.
Keyword(s):
Acid-base regulation; Bottles or small containers/Aquaria (<20 L); Chromista; Coscinodiscus wailesii; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Single species
Supplement to:
Liu, Fengjie; Gledhill, Martha; Tan, Qiaoguo; Zhu, Kechen; Zhang, Qiong; Salaün, Pascal; Tagliabue, Alessandro; Zhang, Yanjun; Weiss, Dominik J; Achterberg, Eric Pieter; Korchev, Yuri (2022): Phycosphere pH of unicellular nano- and micro- phytoplankton cells and consequences for iron speciation. The ISME Journal, 16(10), 2329-2336, https://doi.org/10.1038/s41396-022-01280-1
Original version:
Liu, Fengjie (2022): Data for the phycosphere pH of unicellular nano- and micro- phytoplankton cells and consequences for iron speciation. figshare, https://doi.org/10.6084/m9.figshare.19576477.v1
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-11-25.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeLiu, FengjieStudy
2Species, unique identificationSpecies UIDLiu, Fengjie
3FigureFigLiu, Fengjie
4TreatmentTreatLiu, Fengjie
5Proton gradientsΔ [H+]nmol/lLiu, Fengjiedecrease of H+ concentration in the phycosphere
6ThicknessThickµmLiu, FengjiepH boundary layer
7Temperature, waterTemp°CLiu, Fengjie
8SalinitySalLiu, Fengjie
9Alkalinity, totalATµmol/kgLiu, Fengjie
10Carbon, inorganic, dissolvedDICµmol/kgLiu, Fengjie
11Hydrogen ion concentrationH+nmol/lLiu, Fengjie
12Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
13pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
14Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
15Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
16Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
17Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
18Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
19Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
20Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3286 data points

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