Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (2012): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.778124, Supplement to: Machado, C et al. (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590, hdl:10013/epic.38971.d002
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Abstract:
The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to determine whether N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951 are different or whether they are the same species with morphological, physiological and behavioural variability. N. rossii was used as control. Mitochondrial DNA (mtDNA) was isolated from muscle specimens of N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii, which were collected in Admiralty Bay, King George Island. The DNA was used to amplify a fragment (690 base pairs) of the mitochondrial gene coding region of NADH dehydrogenase subunit 2. Further, the amplicon was digested with the following restriction enzymes: DdeI, HindIII and RsaI. The results showed a variation of the digestion pattern of the fragment amplified between N. rossii, and N. coriiceps Richardson, 1844 or N. neglecta Nybelin, 1951. However, no differences were found between N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951, on the grounds of the same genetic pattern shown by the two fish.
Related to:
Barrera-Oro, Esteban; Eastman, Joseph T; Moreira, Eugenia (2012): Phenotypic plasticity in the Antarctic nototheniid fish Trematomus newnesi: a guide to the identification of typical, large mouth and intermediate morphs. Polar Biology, 35(7), 1047-1056, https://doi.org/10.1007/s00300-011-1152-5
Oleinik, Alla G; Skurikhina, Lubov A; Brykov, Vladimir A (2007): Divergence of Salvelinus species from northeastern Asia based on mitochondrial DNA. Ecology of Freshwater Fish, 16(1), 87-98, https://doi.org/10.1111/j.1600-0633.2006.00187.x
Oleinik, Alla G; Skurikhina, Lubov A; Frolov, Sergei V; Brykov, Vladimir A; Chereshnev, Igor A (2004): Differences Between two Subspecies of Dolly Varden, Salvelinus malma, Revealed by RFLP—PCR Analysis of Mitochondrial DNA. Environmental Biology of Fishes, 69(1-4), 449-459, https://doi.org/10.1023/B:EBFI.0000022897.26755.da
Comment:
Figure 1 in png-format at hdl:10013/epic.38971.d007 and pdf-format at hdl:10013/epic.38971.d006.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Characteristic | Characteristic | Machado, Cintia | |||
| 2 | Notothenia rossii | N. rossii | Machado, Cintia | |||
| 3 | Notothenia coriiceps | N. coriiceps | Machado, Cintia | |||
| 4 | Notothenia neglecta | N. neglecta | Machado, Cintia |
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