Citation:

PANGAEA.913444

Name: Seawater carbonate chemistry and biochemical composition of the coccolithophore Emiliania huxleyi
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Single species

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Abstract:

Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. The diploid stage was shown to contain vastly more osmolytes and mannitol than the haploid stage, which in turn had a higher relative content of amino acids, especially aromatic ones. Besides the differences between the investigated cell types and the general effects on biomass buildup, our analyses indicate that OA imposes only negligible effects on E. huxleyi's biomass composition.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Single species

Supplement to:

Heidenreich, Elena; Wördenweber, Robin; Kirschhöfer, Frank; Nusser, Michael; Friedrich, Frank; Fahl, Kirsten; Kruse, Olaf; Rost, Björn; Franzreb, Matthias; Brenner-Weiß, Gerald; Rokitta, Sebastian D (2019): Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. PLoS ONE, 14(7), e0218564, https://doi.org/10.1371/journal.pone.0218564

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-03-06.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Heidenreich, Elena		study
2	Species	Species		Heidenreich, Elena
3	Registration number of species	Reg spec no		Heidenreich, Elena
4	Uniform resource locator/link to reference	URL ref		Heidenreich, Elena		WoRMS Aphia ID
5	Treatment	Treat		Heidenreich, Elena
6	Alkenones	Alk	arbitrary units	Heidenreich, Elena
7	Alkenones, standard deviation	Alk std dev	±	Heidenreich, Elena
8	Temperature, water	Temp	°C	Heidenreich, Elena		alkenone-derived
9	Temperature, water, standard deviation	Temp std dev	±	Heidenreich, Elena		alkenone-derived
10	Growth rate	µ	1/day	Heidenreich, Elena
11	Growth rate, standard deviation	µ std dev	±	Heidenreich, Elena
12	Cell, diameter	Cell diam	µm	Heidenreich, Elena
13	Cell, diameter, standard deviation	Cell diam std dev	±	Heidenreich, Elena
14	Name	Name		Heidenreich, Elena		substance
15	Category	Cat		Heidenreich, Elena		class and unit
16	Concentration	Conc	arbitrary units	Heidenreich, Elena		per cell
17	Concentration, standard deviation	Conc std dev	±	Heidenreich, Elena		per cell
18	Concentration	Conc	arbitrary units	Heidenreich, Elena		per POC
19	Concentration, standard deviation	Conc std dev	±	Heidenreich, Elena		per POC
20	Salinity	Sal		Heidenreich, Elena
21	Temperature, water	Temp	°C	Heidenreich, Elena
22	pH	pH		Heidenreich, Elena		NBS scale, at the beginning
23	pH, standard deviation	pH std dev	±	Heidenreich, Elena		NBS scale, at the beginning
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
25	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
26	Alkalinity, total	AT	µmol/kg	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
27	Alkalinity, total, standard deviation	AT std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Heidenreich, Elena		at the beginning
29	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Heidenreich, Elena		at the beginning
30	Bicarbonate ion	[HCO3]-	µmol/kg	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
31	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
32	Carbonate ion	[CO3]2-	µmol/kg	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
33	Carbonate ion, standard deviation	[CO3]2- std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the beginning
34	pH	pH		Heidenreich, Elena		NBS scale, at the end
35	pH, standard deviation	pH std dev	±	Heidenreich, Elena		NBS scale, at the end
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Heidenreich, Elena	Calculated using CO2SYS	at the end
37	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the end
38	Alkalinity, total	AT	µmol/kg	Heidenreich, Elena	Calculated using CO2SYS	at the end
39	Alkalinity, total, standard deviation	AT std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the end
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Heidenreich, Elena		at the end
41	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Heidenreich, Elena		at the end
42	Bicarbonate ion	[HCO3]-	µmol/kg	Heidenreich, Elena	Calculated using CO2SYS	at the end
43	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the end
44	Carbonate ion	[CO3]2-	µmol/kg	Heidenreich, Elena	Calculated using CO2SYS	at the end
45	Carbonate ion, standard deviation	[CO3]2- std dev	±	Heidenreich, Elena	Calculated using CO2SYS	at the end
46	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, at the beginning
48	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
49	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
50	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
51	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
52	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
53	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
54	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
55	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the beginning
56	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, at the end
57	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
58	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
59	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
60	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
61	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
62	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
63	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end
64	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

65256 data points

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