Rehder, Linda; Rost, Björn; Rokitta, Sebastian D (2023): Temperature effects on the physiology of photosynthesis and respiration in Phaeodactylum tricornutum [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960034
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Abstract:
Phaeodactylum tricornutum strain CCAP 1052/1A was cultivated at 6°C and 15°C under controlled conditions (32 salinity, F/2 medium, 400 µatm pCO2, 100 µmol photons m-2 s-2 light intentsity 16:8 light:dark cycle) in semi-continous batch cultures. We assessed the carbonate chemistry (pH, total alkalinity, dissolved inorganic carbon), growth rates, particulate organic carbon and nitrogen (POC and PON), chlorophyll a quota (Chl a), POC:PON ratios, Chl a:POC ratios as well as production rates at both acclimation temperatures. Additionally, we performed biological invivo assays to measure rates of gross photosynthetic oxygen release, gross photosynthetic carbon uptake, respiratory oxygen uptake and respiratory carbon release using membrane-inlet mass-spectrometry. Assays were performed in photosynthesis-irradiance-(PI-)curves of increasing light intensity (0, 50, 150, 250, 400 µmol photons m-2 s-2). First rates were measured under acclimation temperature (6°C and 15°C), directly afterwards, the assay temperature was abruptly shifted to 15°C or 6°C, respectively, and the PI-curve measurement was repeated, so that 6°C acclimated cells were measured at 15°C and 15°C acclimated cells were measured at 6°C. Q10 factors were calculated from acclimated cells und the respective temperature shift. Photosynthetic and respiratory quotients were calculated for acclimated cells as well as after the abrupt temperature shift. PI-parameters, i.e. maximum photosynthesis rate, light use efficiency and light saturation index were calculated. All experiments were performed in laboratories at the Alfred-Wegener-Institute Bremerhaven.
Keyword(s):
Supplement to:
Rehder, Linda; Rost, Björn; Rokitta, Sebastian D (2023): Abrupt and acclimation responses to changing temperature elicit divergent physiological effects in the diatom Phaeodactylum tricornutum. New Phytologist, 239(3), 1005-1013, https://doi.org/10.1111/nph.18982
References:
Guillard, R R L; Ryther, J H (1962): Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Canadian Journal of Microbiology, 8(2), 229-239, https://doi.org/10.1139/m62-029
Knap, Anthony H; Michaels, A; Close, A R; Ducklow, Hugh W; Dickson, Andrew G (1996): Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS, Reprint of the IOC Manuals and Guides No. 29, UNESCO 1994, 19, 210 pp, hdl:10013/epic.27912.d001
Rokitta, Sebastian D; Rost, Björn (2012): Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 57(2), 607-618, https://doi.org/10.4319/lo.2012.57.2.0607
Additional metadata:
Rehder, Linda (2023): Detailed parameter information for Phaeodactylum tricornutum data. Pangaea_Parameter_Rehder_et_al.csv
Comment:
Phaeodactylum tricornutum strain CCAP 1052/1A was originally sampled in 2003 from an estuary, polluted water (industrial area and seaside resort) Blackpool, England (detailed information from Culture Collection of algae and protozoa; https://www.ccap.ac.uk/catalogue/strain-1055-1). Prior to the experiments, the culture was at least one year cultivated at 15°C in North Sea water enriched with F/2 medium under 16:8 light:dark irradiance of ~10 µmol photons m-2 s-1 with regular dilution every ~4 weeks.
Experiment 1A and 1B refer to 'bulk' parameter measurements, i.e. growth rates, biomass and pigmentation. Due to logistical issues, Exp_1A and Exp_1B are from different batches, but the same stock culture. Exp_2 is the same batch as Exp_1A and refers to physiological measurements. Temperature shifts only apply to Exp_2 and took place after cultures were concentrated and physiological rates were measured under in-situ temperature at four different light levels in the membrane inlet mass spectrometer (MIMS). The temperature shift took place inside the cuvette of the MIMS and took ~1 h.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Rehder, Linda | |||
| 2 | Date/time start, experiment | Date/time start exp | Rehder, Linda | including acclimation time for Exp_1A | ||
| 3 | Date/time end, experiment | Date/time end exp | Rehder, Linda | |||
| 4 | Sampling date/time, experiment | Date/time sampling exp | Rehder, Linda | |||
| 5 | Sample ID | Sample ID | Rehder, Linda | |||
| 6 | Type of study | Study type | Rehder, Linda | |||
| 7 | Laboratory | Lab | Rehder, Linda | |||
| 8 | Species | Species | Rehder, Linda | |||
| 9 | Species, unique identification | Species UID | Rehder, Linda | |||
| 10 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Rehder, Linda | |||
| 11 | Species, unique identification (URI) | Species UID (URI) | Rehder, Linda | |||
| 12 | Strain | Strain | Rehder, Linda | |||
| 13 | Treatment: temperature | T:temp | °C | Rehder, Linda | ||
| 14 | Treatment: light:dark cycle | T:L:D | hh:hh | Rehder, Linda | ||
| 15 | Treatment: light intensity | T:Io | µmol/m2/s | Rehder, Linda | ||
| 16 | Salinity | Sal | Rehder, Linda | |||
| 17 | Medium | Medium | Rehder, Linda | |||
| 18 | Generation | Generation | # | Rehder, Linda | in acclimation | |
| 19 | pH | pH | Rehder, Linda | NBS scale | ||
| 20 | Alkalinity, total | AT | µmol/kg | Rehder, Linda | Titration analyzer, Schott Instruments, TitroLine alpha plus | |
| 21 | Carbon, inorganic, dissolved | DIC | µmol/kg | Rehder, Linda | Measured with colorimetric assay on QuAAtro continuous segmented flow analyzer (Seal Analytical) | |
| 22 | Growth rate | µ | 1/day | Rehder, Linda | Coulter Counter (Beckman Coulter) | |
| 23 | Carbon, organic, particulate, per cell | POC/cell | pg/# | Rehder, Linda | Elemental analyzer, EuroVector, EA 3000 | |
| 24 | Nitrogen, organic, particulate, per cell | PON/cell | pg/# | Rehder, Linda | Elemental analyzer, EuroVector, EA 3000 | |
| 25 | Carbon, organic, particulate/Nitrogen, organic, particulate ratio | POC/PON | mol/mol | Rehder, Linda | Calculated | |
| 26 | Particulate organic carbon production per cell | POC prod/cell | pg/#/day | Rehder, Linda | Calculated | µ*POC |
| 27 | Chlorophyll a per cell | Chl a/cell | pg/# | Rehder, Linda | Laboratory fluorometer, Turner, Trilogy | |
| 28 | Chlorophyll a/particulate organic carbon ratio | Chl a/POC | Rehder, Linda | Calculated | pg/pg | |
| 29 | Respiratory oxygen uptake rate, per chlorophyll a | RO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | RO2_accl: rate of respiratory O2 uptake under acclimation temperature |
| 30 | Respiratory oxygen uptake rate, per chlorophyll a | RO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | RO2_shifted: rate of respiratory O2 uptake after abrupt temperature shift |
| 31 | Factor quantifying temperature dependent change of rates of processes | Q10 | Rehder, Linda | Calculation according to Rehder et al. (2023) | Q10 of RO2, calculated from RO2_accl and RO2_shifted | |
| 32 | Respiratory carbon release rate, per chlorophyll a | RCO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | RCO2_accl: rate of respiratory CO2 release under acclimation temperature |
| 33 | Respiratory carbon release rate, per chlorophyll a | RCO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | RCO2_shifted: rate of respiratory CO2 release after abrupt temperature shift |
| 34 | Factor quantifying temperature dependent change of rates of processes | Q10 | Rehder, Linda | Calculation according to Rehder et al. (2023) | Q10 of RO2, calculated from RO2_accl and RO2_shifted | |
| 35 | Respiratory quotient | RQ | Rehder, Linda | Calculation according to Rehder et al. (2023) | after acclimation | |
| 36 | Respiratory quotient | RQ | Rehder, Linda | Calculation according to Rehder et al. (2023) | after the abrupt temperature shift | |
| 37 | Gross photosynthetic oxygen release rate, per chlorophyll a | PSO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSO2_accl_V150: rate of photosynthetic O2 release under acclimation temperature at in-situ light intensity |
| 38 | Gross photosynthetic oxygen release rate, per chlorophyll a | PSO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSO2_shifted_V150: rate of photosynthetic O2 release after abrupt temperature shift at in-situ light intensity |
| 39 | Factor quantifying temperature dependent change of rates of processes | Q10 | Rehder, Linda | Calculation according to Rehder et al. (2023) | Q10 of PSO2 at in-situ light intensity, calculated from PSO2_accl_V150 and PSO2_shifted_V150 | |
| 40 | Gross photosynthetic carbon uptake rate, per chlorophyll a | PSCO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSCO2_accl_V150: rate of photosynthetic C uptake under acclimation temperature at in-situ light intensity |
| 41 | Gross photosynthetic carbon uptake rate, per chlorophyll a | PSCO2 | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSCO2_shifted_V150: rate of photosynthetic C uptake after abrupt temperature shift at in-situ light intensity |
| 42 | Factor quantifying temperature dependent change of rates of processes | Q10 | Rehder, Linda | Calculation according to Rehder et al. (2023) | Q10 of PSO2 at in-situ light intensity, calculated from PSO2_accl_V150 and PSO2_shifted_V150 | |
| 43 | Photosynthetic quotient | PQ | Rehder, Linda | Calculation according to Rehder et al. (2023) | under acclimation temperature at in-situ light intensity | |
| 44 | Photosynthetic quotient | PQ | Rehder, Linda | Calculation according to Rehder et al. (2023) | after abrupt temperature shift at in-situ light intensity | |
| 45 | Maximum photosynthetic oxygen release rate, per chlorophyll a | PSO2 Vmax | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSO2_accl_Vmax: maximum rate of photosynthetic O2 release under acclimation temperature |
| 46 | Maximum photosynthetic oxygen release rate, per chlorophyll a | PSO2 Vmax | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSO2_shifted_Vmax: maximum rate of photosynthetic O2 releaseafter abrupt temperature shift |
| 47 | Factor quantifying temperature dependent change of rates of processes | Q10 | Rehder, Linda | Calculation according to Rehder et al. (2023) | Q10 of maximum PSO2, calculated PSO2_accl_Vmax and PSO2_shifted_Vmax | |
| 48 | Maximum photosynthetic carbon uptake rate, per chlorophyll a | PSCO2 Vmax | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSCO2_accl_Vmax: maximum rate of photosynthetic C uptake under acclimation temperature |
| 49 | Maximum photosynthetic carbon uptake rate, per chlorophyll a | PSCO2 Vmax | µmol/mg/h | Rehder, Linda | Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime | PSCO2_shifted_Vmax: maximum rate of photosynthetic C uptake after abrupt temperature shift |
| 50 | Factor quantifying temperature dependent change of rates of processes | Q10 | Rehder, Linda | Calculation according to Rehder et al. (2023) | Q10 of maximum PSCO2, calculated PSCO2_accl_Vmax and PSCO2_shifted_Vmax | |
| 51 | Light saturation index | Ik | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | Ik of PSO2 under acclimation temperature |
| 52 | Light saturation index | Ik | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | Ik of PSO2 after abrupt temperature shift |
| 53 | Light use efficiency | alpha | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | alpha of PSO2 under acclimation temperature |
| 54 | Light use efficiency | alpha | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | alpha of PSO2 after abrupt temperature shift |
| 55 | Light saturation index | Ik | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | Ik of PSCO2 under acclimation temperature |
| 56 | Light saturation index | Ik | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | Ik of PSCO2 after abrupt temperature shift |
| 57 | Light use efficiency | alpha | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | alpha of PSCO2 under acclimation temperature |
| 58 | Light use efficiency | alpha | µmol/m2/s | Rehder, Linda | Calculation according to Rokitta & Rost (2012) | alpha of PSCO2 after abrupt temperature shift |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
736 data points