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Eichner, Meri; Thoms, Silke; Kranz, Sven A; Rost, Björn (2015): Seawater carbonate chemistry and cellular inorganic carbon fluxes in Trichodesmium. PANGAEA, https://doi.org/10.1594/PANGAEA.956021

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Abstract:
To predict effects of climate change on phytoplankton, it is crucial to understand how their mechanisms for carbon acquisition respond to environmental conditions. Aiming to shed light on the responses of extra- and intracellular inorganic C (Ci) fluxes, the cyanobacterium Trichodesmium erythraeum IMS101 was grown with different nitrogen sources (N2 vs NO3 –) and pCO2 levels (380 vs 1400 µatm). Cellular Ci fluxes were assessed by combining membrane inlet mass spectrometry (MIMS), 13C fractionation measurements, and modelling. Aside from a significant decrease in Ci affinity at elevated pCO2 and changes in CO2 efflux with different N sources, extracellular Ci fluxes estimated by MIMS were largely unaffected by the treatments. 13C fractionation during biomass production, however, increased with pCO2, irrespective of the N source. Strong discrepancies were observed in CO2 leakage estimates obtained by MIMS and a 13C-based approach, which further increased under elevated pCO2. These offsets could be explained by applying a model that comprises extracellular CO2 and HCO3– fluxes as well as internal Ci cycling around the carboxysome via the CO2 uptake facilitator NDH-14. Assuming unidirectional, kinetic fractionation between CO2 and HCO3– in the cytosol or enzymatic fractionation by NDH-14, both significantly improved the comparability of leakage estimates. Our results highlight the importance of internal Ci cycling for 13C composition as well as cellular energy budgets of Trichodesmium, which ought to be considered in process studies on climate change effects.
Keyword(s):
Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium erythraeum
Supplement to:
Eichner, Meri; Thoms, Silke; Kranz, Sven A; Rost, Björn (2015): Cellular inorganic carbon fluxes in Trichodesmium: a combined approach using measurements and modelling. Journal of Experimental Botany, 66(3), 749-759, https://doi.org/10.1093/jxb/eru427
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-02-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeEichner, MeriStudy
2SpeciesSpeciesEichner, Meri
3TreatmentTreatEichner, Meri
4Time point, descriptiveTime pointEichner, Meri
5Carbon fixation rate, per chlorophyll aC fix/Chl aµmol/mg/hEichner, MeriNet
6Carbon fixation rate, standard deviationC fix std dev±Eichner, MeriNet
7Gross carbon uptake rate, per chlorophyll aG C upt rate/Chl aµmol/mg/hEichner, Meri
8Carbon uptake rate, standard deviationC upt rate std dev±Eichner, MeriGross
9Bicarbonate uptake rate, per chlorophyll aHCO3 upt rate/Chlµmol/mg/hEichner, Meri
10Bicarbonate uptake in chlorophyll, standard deviationHCO3 upt/Chl std dev±Eichner, Meri
11Carbon dioxide uptake rate, per chlorophyll aCO2 upt rate/Chlµmol/mg/hEichner, MeriGross
12Carbon dioxide uptake, per chlorophyll, standard deviationCO2 upt/Chl std dev±Eichner, MeriGross
13Carbon dioxide uptake rate, per chlorophyll aCO2 upt rate/Chlµmol/mg/hEichner, MeriNet
14Carbon dioxide uptake, per chlorophyll, standard deviationCO2 upt/Chl std dev±Eichner, MeriNet
15RatioRatioEichner, Meri[HCO3]-:Ci uptake
16Ratio, standard deviationRatio std dev±Eichner, Meri[HCO3]-:Ci uptake
17Carbon dioxide efflux, per chlorophyll aCO2 efflux/Chl aµmol/mg/hEichner, Meri
18Carbon dioxide efflux, per chlorophyll a, standard deviationCO2 efflux/Chl a std dev±Eichner, Meri
19RatioRatioEichner, Merileakage (CO2 efflux/gross Ci uptake)
20Ratio, standard deviationRatio std dev±Eichner, Merileakage (CO2 efflux/gross Ci uptake)
21RatioRatioEichner, Merileakage (CO2 efflux/gross Ci uptake) based on MIMS measurements
22Ratio, standard deviationRatio std dev±Eichner, Merileakage (CO2 efflux/gross Ci uptake) based on MIMS measurements
23RatioRatioEichner, Merileakage (CO2 efflux/gross Ci uptake) based on 13C fractionation
24Ratio, standard deviationRatio std dev±Eichner, Merileakage (CO2 efflux/gross Ci uptake) based on 13C fractionation
25SalinitySalEichner, Meri
26Temperature, waterTemp°CEichner, Meri
27pHpHEichner, MeriPotentiometricNBS scale
28pH, standard deviationpH std dev±Eichner, MeriPotentiometricNBS scale
29Alkalinity, totalATµmol/kgEichner, MeriPotentiometric titration
30Alkalinity, total, standard deviationAT std dev±Eichner, MeriPotentiometric titration
31Carbon, inorganic, dissolvedDICµmol/kgEichner, MeriCalculated using CO2SYS
32Carbon, inorganic, dissolved, standard deviationDIC std dev±Eichner, MeriCalculated using CO2SYS
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmEichner, MeriCalculated using CO2SYS
34Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Eichner, MeriCalculated using CO2SYS
35Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
37Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
580 data points

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