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Rahlff, Janina; Giebel, Helge-Ansgar; Lehners, Carola; Ribas-Ribas, Mariana; Wurl, Oliver; Bergström, Kristofer; Holmfeldt, Karin (2023): Natural sea surface slicks from the Baltic Sea investigated for cell and virus-like particle abundance, surfactants and dissolved organic carbon in May 2021 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.955904

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Abstract:
Flow cytometry samples for the measurement of virus-like particles and prokaryotic abundance were sampled from the sea-surface microlayer inside and outside a natural surface slick and respective underlying water samples at three sites in the Kalmar Sound, near Rockneby, Sweden, using a boat. A glass plate sampler and a Ruttner sampler were used to sample the sea-surface microlayer and underlying water from 70 cm depth, respectively. For site 1, dissolved organic carbon and surfactants were additionally measured in all four sample types. Samples for flow cytometry were fixed with glutardialdehyde (0.5% final concentration). Some metadata for wind speed, temperature and salinity were collected. These data complement metagenomic analysis of viral communities in the sea-surface microlayer of a natural surface slick (Rahlff et al., unpublished).
Keyword(s):
flow cytometry; neuston; sea-surface microlayer; surface water; Surfactants; Viruses
Supplement to:
Rahlff, Janina; Wietz, Matthias; Giebel, Helge-Ansgar; Bayfield, Oliver; Nilsson, Emelie; Bergström, Kristofer; Kieft, Kristopher; Anantharaman, Karthik; Ribas-Ribas, Mariana; Schweitzer, Hannah D; Wurl, Oliver; Hoetzinger, Matthias; Antson, Alfred; Holmfeldt, Karin (2023): Ecogenomics and cultivation reveal distinctive viral-bacterial communities in the surface microlayer of a Baltic Sea slick. ISME Communications, 3, 97, https://doi.org/10.1038/s43705-023-00307-8
References:
Martínez-García, Sandra; Bunse, Carina; Pontiller, Benjamin; Baltar, Federico; Israelsson, Stina; Fridolfsson, Emil; Lindh, Markus V; Lundin, Daniel; Legrand, Catherine; Pinhassi, Jarone (2022): Seasonal Dynamics in Carbon Cycling of Marine Bacterioplankton Are Lifestyle Dependent. Frontiers in Microbiology, 13, 834675, https://doi.org/10.3389/fmicb.2022.834675
Funding:
German Research Foundation (DFG), grant/award no. 446702140: VIBOCAT - Erforschung des Virioneustons: Viral-bakterielle Interaktionen zwischen Ozean und Atmosphäre
Coverage:
Median Latitude: 56.837553 * Median Longitude: 16.467317 * South-bound Latitude: 56.832660 * West-bound Longitude: 16.447770 * North-bound Latitude: 56.841470 * East-bound Longitude: 16.479770
Date/Time Start: 2021-05-31T07:10:00 * Date/Time End: 2021-05-31T09:50:00
Minimum DEPTH, water: 0.001 m * Maximum DEPTH, water: 0.700 m
Event(s):
Kalmarsund_1 * Latitude Start: 56.832660 * Longitude Start: 16.447770 * Latitude End: 56.832690 * Longitude End: 16.448530 * Date/Time Start: 2021-05-31T07:10:00 * Date/Time End: 2021-05-31T08:10:00 * Location: Kalmar Strait * Method/Device: Multiple investigations (MULT)
Kalmarsund_2 * Latitude Start: 56.841470 * Longitude Start: 16.473450 * Latitude End: 56.841280 * Longitude End: 16.474610 * Date/Time Start: 2021-05-31T08:30:00 * Date/Time End: 2021-05-31T09:20:00 * Location: Kalmar Strait * Method/Device: Multiple investigations (MULT)
Kalmarsund_3 * Latitude: 56.838610 * Longitude: 16.479770 * Date/Time Start: 2021-05-31T09:30:00 * Date/Time End: 2021-05-31T09:50:00 * Location: Kalmar Strait * Method/Device: Multiple investigations (MULT)
Comment:
The virus-like particles and prokaryote abundance samples had one unwanted freeze thaw cycle due to shipment problems. At best, this data set should not be compared with others, just comparisons within this dataset should be done.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventRahlff, Janina
2Station labelStationRahlff, Janina
3Sample typeSamp typeRahlff, Janina
4DATE/TIMEDate/TimeRahlff, JaninaGeocode – start (UTC)
5DATE/TIMEDate/TimeRahlff, JaninaGeocode – end (UTC)
6Date/Time localDate/Time localRahlff, Janinastart
7Date/Time localDate/Time localRahlff, Janinaend
8LATITUDELatitudeRahlff, JaninaGeocode
9LONGITUDELongitudeRahlff, JaninaGeocode
10Virus-like particlesVirus-like#/mlRahlff, JaninaFlow cytometer, BD Biosciences, BD Accuri C6
11ProkaryotesProkaryotes#/mlRahlff, JaninaFlow cytometer, BD Biosciences, BD Accuri C6Prokaryotic cells
12SurfactantsSurfactµg/lRahlff, JaninaVoltammetry stand, Metrohm, 747 VA Computraceµg Teq L⁻¹ (Teq = Triton-X-100 equivalent)
13Carbon, organic, dissolvedDOCmg/lRahlff, JaninaDissolved organic carbon analysis according to Martínez-García et al. (2022)
14HabitatHabitatRahlff, Janina
15DEPTH, waterDepth watermRahlff, JaninaGeocode
16Sample methodSample methodRahlff, Janina
17Wind speedffm/sRahlff, JaninaDigital anemometer, MASTECH, MS6252Aduring sampling
18Temperature, waterTemp°CRahlff, JaninaMulti-parameter portable meter, Xylem Analytics, MultiLine® Multi 3620 IDS
19SalinitySalRahlff, JaninaMulti-parameter portable meter, Xylem Analytics, MultiLine® Multi 3620 IDSSeawater
20CommentCommentRahlff, JaninaNotes
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
138 data points

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