Adam, Nicole; Perner, Mirjam (2021): Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.930105
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Abstract:
Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields.
Supplement to:
Adam, Nicole; Perner, Mirjam (2018): Novel hydrogenases from deep-sea hydrothermal vent metagenomes identified by a recently developed activity-based screen. The ISME Journal, 12(5), 1225-1236, https://doi.org/10.1038/s41396-017-0040-6
Coverage:
Median Latitude: -7.548333 * Median Longitude: -13.031333 * South-bound Latitude: -9.543833 * West-bound Longitude: -13.508833 * North-bound Latitude: -4.804000 * East-bound Longitude: -12.372333
Date/Time Start: 2009-04-17T18:08:00 * Date/Time End: 2009-04-30T11:50:00
Minimum Elevation: -2980.0 m * Maximum Elevation: -1519.0 m
Event(s):
M78/2_274 * Latitude: -4.804000 * Longitude: -12.372333 * Date/Time: 2009-04-17T18:08:00 * Elevation: -2980.0 m * Campaign: M78/2 (MARSUED5) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV)
M78/2_314 * Latitude: -8.297167 * Longitude: -13.508833 * Date/Time: 2009-04-27T10:01:00 * Elevation: -2886.0 m * Campaign: M78/2 (MARSUED5) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV)
M78/2_324 * Latitude: -9.543833 * Longitude: -13.212833 * Date/Time: 2009-04-30T11:50:00 * Elevation: -1519.0 m * Campaign: M78/2 (MARSUED5) * Basis: Meteor (1986) * Method/Device: Remote operated vehicle (ROV)
Comment:
Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity. The hydrogen upake activity of the fosmid clones has been determined as described in the cited publication.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Perner, Mirjam | |||
| 2 | Area/locality | Area | Perner, Mirjam | |||
| 3 | Sample ID | Sample ID | Perner, Mirjam | Clone | ||
| 4 | Hydrogen uptake activity | H2 upt act | nmol/min/mg | Perner, Mirjam | at 25°C | |
| 5 | Hydrogen uptake activity, standard deviation | H2 upt act std dev | ± | Perner, Mirjam | at 25°C | |
| 6 | Hydrogen uptake activity | H2 upt act | nmol/min/mg | Perner, Mirjam | at 55°C | |
| 7 | Hydrogen uptake activity, standard deviation | H2 upt act std dev | ± | Perner, Mirjam | at 55°C | |
| 8 | Accession number, genetics | Access no gen | Perner, Mirjam | |||
| 9 | Sample method | Sample method | Perner, Mirjam |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
32 data points