Strobel, Anneli; Leo, Elettra; Pörtner, Hans-Otto; Mark, Felix Christopher (2014): Experiment: Elevated temperature and PCO2 affect enzyme activities in differentially oxidative tissues of Notothenia rossii [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.829831, Supplement to: Strobel, A et al. (2013): Elevated temperature and PCO2 shift metabolic pathways in differentially oxidative tissues of Notothenia rossii. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 166(1), 48-57, https://doi.org/10.1016/j.cbpb.2013.06.006
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Abstract:
Mitochondrial plasticity plays a central role in setting the capacity for acclimation of aerobic metabolism in ectotherms in response to environmental changes. We still lack a clear picture if and to what extent the energy metabolism and mitochondrial enzymes of Antarctic fish can compensate for changing temperatures or PCO2 and whether capacities for compensation differ between tissues. We therefore measured activities of key mitochondrial enzymes (citrate synthase (CS), cytochrome c oxidase (COX)) from heart, red muscle, white muscle and liver in the Antarctic fish Notothenia rossii after warm- (7 °C) and hypercapnia- (0.2 kPa CO2) acclimation vs. control conditions (1 °C, 0.04 kPa CO2). In heart, enzymes showed elevated activities after cold-hypercapnia acclimation, and a warm-acclimation-induced upward shift in thermal optima. The strongest increase in enzyme activities in response to hypercapnia occurred in red muscle. In white muscle, enzyme activities were temperature-compensated. CS activity in liver decreased after warm-normocapnia acclimation (temperature-compensation), while COX activities were lower after cold- and warm-hypercapnia exposure, but increased after warm-normocapnia acclimation. In conclusion, warm-acclimated N. rossii display low thermal compensation in response to rising energy demand in highly aerobic tissues, such as heart and red muscle. Chronic environmental hypercapnia elicits increased enzyme activities in these tissues, possibly to compensate for an elevated energy demand for acid-base regulation or a compromised mitochondrial metabolism, that is predicted to occur in response to hypercapnia exposure. This might be supported by enhanced metabolisation of liver energy stores. These patterns reflect a limited capacity of N. rossii to reorganise energy metabolism in response to rising temperature and PCO2.
Further details:
Strobel, Anneli; Leo, Elettra; Pörtner, Hans-Otto; Mark, Felix Christopher (2013): Seawater carbonate chemistry, citrate synthase (CS), cytochrome c oxidase (COX) of the Antarctic fish Notothenia rossii in a laboratory experiment. PANGAEA, https://doi.org/10.1594/PANGAEA.831182
Project(s):
Funding:
German Research Foundation (DFG), grant/award no. 5472008: Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas
Coverage:
Latitude: -62.233330 * Longitude: -58.666660
Minimum Elevation: -35.0 m * Maximum Elevation: -35.0 m
Event(s):
PotterCove * Latitude: -62.233330 * Longitude: -58.666660 * Elevation: -35.0 m * Location: Potter Cove, King George Island, Antarctic Peninsula * Campaign: Jubany_Dallmann * Basis: Carlini/Jubany Station * Method/Device: Multiple investigations (MULT)
Comment:
Notothenia rossii acclimated to higher temperature and PCO2; Citrate synthase (CS) and cytochrome c oxidase (COX) activities are given in nmol per minute and mg protein (CS/protein; COX/protein) or mg tissue fresh mass (CS/MW; COX/MW) at the assay temperatures of 0, 6, 9, 12 °C in heart, liver, red muscle & white muscle tissue of all acclimated animals and the control.
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Identification | ID | Strobel, Anneli | No. of experimental animal | ||
2 | Species | Species | Strobel, Anneli | |||
3 | Temperature, technical | T tech | °C | Strobel, Anneli | Acclimation temperature | |
4 | Carbon dioxide, partial pressure | pCO2 | ppmv | Strobel, Anneli | Acclimation PCO2 | |
5 | Temperature, technical | T tech | °C | Strobel, Anneli | Enzyme assay temperature | |
6 | Citrate synthase activity, per protein | CS act/protein | nmol/min/mg | Strobel, Anneli | Tissue: heart | |
7 | Cytochrome c oxidase activity, per protein | COX act/protein | nmol/min/mg | Strobel, Anneli | Tissue: heart | |
8 | Citrate synthase activity, per protein | CS act/protein | nmol/min/mg | Strobel, Anneli | Tissue: liver | |
9 | Cytochrome c oxidase activity, per protein | COX act/protein | nmol/min/mg | Strobel, Anneli | Tissue: liver | |
10 | Citrate synthase activity, per protein | CS act/protein | nmol/min/mg | Strobel, Anneli | Tissue: red muscle | |
11 | Cytochrome c oxidase activity, per protein | COX act/protein | nmol/min/mg | Strobel, Anneli | Tissue: red muscle | |
12 | Citrate synthase activity, per protein | CS act/protein | nmol/min/mg | Strobel, Anneli | Tissue: white muscle | |
13 | Cytochrome c oxidase activity, per protein | COX act/protein | nmol/min/mg | Strobel, Anneli | Tissue: white muscle | |
14 | Citrate synthase activity per fresh mass | CS act/fm | nmol/min/mg | Strobel, Anneli | Tissue: heart | |
15 | Cytochrome c oxidase activity, per fresh mass | COX act/fm | nmol/min/mg | Strobel, Anneli | Tissue: heart | |
16 | Citrate synthase activity per fresh mass | CS act/fm | nmol/min/mg | Strobel, Anneli | Tissue: liver | |
17 | Cytochrome c oxidase activity, per fresh mass | COX act/fm | nmol/min/mg | Strobel, Anneli | Tissue: liver | |
18 | Citrate synthase activity per fresh mass | CS act/fm | nmol/min/mg | Strobel, Anneli | Tissue: red muscle | |
19 | Cytochrome c oxidase activity, per fresh mass | COX act/fm | nmol/min/mg | Strobel, Anneli | Tissue: red muscle | |
20 | Citrate synthase activity per fresh mass | CS act/fm | nmol/min/mg | Strobel, Anneli | Tissue: white muscle | |
21 | Cytochrome c oxidase activity, per fresh mass | COX act/fm | nmol/min/mg | Strobel, Anneli | Tissue: white muscle |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
2003 data points
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