Hu, Marian Y; Sucre, Elliott; Charmantier-Daures, Mireille; Charmantier, Guy; Lucassen, Magnus; Himmerkus, Nina; Melzner, Frank (2015): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.842790, Supplement to: Hu, MY et al. (2010): Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods. Cell and Tissue Research, 339(3), 571-583, https://doi.org/10.1007/s00441-009-0921-8
Always quote citation above when using data! You can download the citation in several formats below.
Published: 2015-02-13 • DOI registered: 2015-04-17
Abstract:
The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species: the squid Loligo vulgaris and the cuttlefish Sepia officinalis. This is the first report of the immunohistochemical localization of cephalopod Na+/K+-ATPase with the polyclonal antibody alpha (H-300) raised against the human alpha1-subunit of Na+/K+-ATPase. Na+/K+-ATPase immunoreactivity was observed in several tissues (gills, pancreatic appendages, nerves), exclusively located in baso-lateral membranes lining blood sinuses. Furthermore, large single cells in the gill of adult L. vulgaris specimens closely resembled Na+/K+-ATPase-rich cells described in fish. Immunohistochemical observations indicated that the amount and distribution of Na+/K+-ATPase in late cuttlefish embryos was similar to that found in juvenile and adult stages. The ion-regulatory epithelia (e.g., gills, excretory organs) of the squid embryos and paralarvae exhibited less differentiation than adults. Na+/K+-ATPase activities for whole animals were higher in hatchlings of S. officinalis (157.0 ± 32.4 µmol/g FM/h) than in those of L. vulgaris (31.8 ± 3.3 µmol/g FM/h). S. officinalis gills and pancreatic appendages achieved activities of 94.8 ± 18.5 and 421.8 ± 102.3 µmol ATP/g FM/h, respectively. High concentrations of Na+/K+-ATPase in late cephalopod embryos might be important in coping with the challenging abiotic conditions (low pH, high pCO2) that these organisms encounter inside their eggs. Our results also suggest a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.
Project(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species | Species | Hu, Marian Y | |||
| 2 | Type | Type | Hu, Marian Y | |||
| 3 | Replicates | Repl | # | Hu, Marian Y | ||
| 4 | Sodium/Potassium adenosine triphosphatase activity | Na/K-ATPase act | µmol/g/h | Hu, Marian Y | ||
| 5 | Standard deviation | Std dev | ± | Hu, Marian Y | Na/K-ATPase | |
| 6 | Adenosine triphosphatase activity | ATPase act | µmol/g/h | Hu, Marian Y | other ATPases | |
| 7 | Standard deviation | Std dev | ± | Hu, Marian Y | other ATPases | |
| 8 | Adenosine triphosphatase activity | ATPase act | µmol/g/h | Hu, Marian Y | total ATPase | |
| 9 | Standard deviation | Std dev | ± | Hu, Marian Y | total ATPase | |
| 10 | Change in Sodium/potassium ATPase alpha subunit expression | NaK-ATPase | % | Hu, Marian Y |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
40 data points
Download Data
Datasets with similar metadata
- Hu, MY; Tseng, Y-C; Stumpp, M et al. (2011): Seawater carbonate chemistry, mass, length and gene expression of Sepia officinalis during experiments, 2011. https://doi.org/10.1594/PANGAEA.774787
- Kreiss, CM; Michael, K; Bock, C et al. (2015): Impact of long-term moderate hypercapnia and elevated temperature on the energy budget of isolated gills and branchial in vivo and in vitro enzyme capacities of Atlantic cod (Gadus morhua). https://doi.org/10.1594/PANGAEA.848599
- Hu, MY; Tseng, Y-C; Lin, L-Y et al. (2015): ATPase activity, proton gradients and proton secretion inhibition during experiments with Sepia officinalis and Sepioteuthis lessoniana. https://doi.org/10.1594/PANGAEA.844555
Users interested in this dataset were also interested in
- Wieczorek, M; Kruse, S; Epp, LS et al. (2017): Field data for larches growing in the Taimyr treeline ecotone. https://doi.org/10.1594/PANGAEA.874612
- Hillenbrand, C-D; Kuhn, G; Smith, JA et al. (2013): (Table DR1) Age determination of sediment cores from the Amundsen Sea. https://doi.org/10.1594/PANGAEA.773979
- Haas, C; Nicolaus, M; Willmes, S et al. (2011): Snow density measured in snow pits using the snow fork during the drift station (2004-11-29 to 2005-01-02) of the POLARSTERN cruise ANT-XXII/2 (ISPOL). https://doi.org/10.1594/PANGAEA.772850