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Kunisch, Erin H; Graeve, Martin; Gradinger, Rolf; Flores, Hauke; Varpe, Øystein; Woll, Matthias; Bluhm, Bodil Annikki (2023): Lipids, fatty acids, and δ13C values in fatty acids measured in Arctic zooplankton and ice amphipod species during polar day (June and July) and polar night (January) in 2017/2018 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.961753

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Abstract:
This dataset consists of lipid analysis, fatty acid analysis, and compound specific stable isotope analysis of δ13C values in fatty acids. These parameters were measured in three pelagic zooplankton (Calanus glacialis, Thysanoessa inermis, and Themisto libellula) and two sea-ice associated amphipods (Apherusa glacialis, Gammarus wilkitzkii). Zooplankton and ice-associated amphipods were collected on the R/V Polarstern PS106/1 (23 May - 21 June 2017) and PS106/2 (23 June - 20 July 2017) campaigns and on two R/V Helmer Hanssen polar night campaigns (4 - 16 January 2017 and 5 - 18 January 2018). See document details for the different types of gear used, which included Multinets, pelagic trawls, Surface Underwater Ice Trawls, and ROV nets. Organisms were collected north of Svalbard, the Barents Sea, and within the Nansen Basin of the Arctic Ocean. Lipid class analysis was conducted using high performance liquid chromatography. Extracted lipids were converted into fatty acid methyl esters, and quantified (via an internal standard) using an Agilent 6890N gas chromatograph. Compound specific stable isotope ratios of fatty acid methyl esters (δ13C fa) were analyzed using a Trace Ultra gas chromatograph (GC), a GC Isolink system, and a Delta V Plus isotope ratios mass spectrometer, connected to a Conflo IV interface. The purpose of these measurements was to compare feeding activity between polar day (June/July) and polar night (January) in these five invertebrate species. Also, in light of recent research that there are higher levels of biological species activity during polar night than previously assumed, determine if these invertebrates were maintaining higher levels of activity on stored lipids, opportunistic feeding, or a combination of both.
Keyword(s):
apherusa glacialis; Arctic; Calanus glacialis; CSIA; fatty acids; Gammarus wilkitzkii; isotope analysis; lipid classes; polar night; sympagic fauna; Themisto libellula; Thysanoessa inermis; Zooplankton
Supplement to:
Kunisch, Erin H; Graeve, Martin; Gradinger, Rolf; Flores, Hauke; Varpe, Øystein; Bluhm, Bodil Annikki (2023): What we do in the dark: Prevalence of omnivorous feeding activity in Arctic zooplankton during polar night. Limnology and Oceanography, 68(8), 1835-1851, https://doi.org/10.1002/lno.12389
Related to:
Macke, Andreas; Flores, Hauke (2018): The Expeditions PS106/1 and 2 of the Research Vessel POLARSTERN to the Arctic Ocean in 2017. Berichte zur Polar- und Meeresforschung = Reports on Polar and Marine Research, 719, 171 pp, https://doi.org/10.2312/BzPM_0719_2018
Funding:
The Research Council of Norway (RCN), grant/award no. 244319: Arctic Ocean ecosystems - Applied technology, Biological interactions and Consequences in an era of abrupt climate change (Arctic ABC)
Coverage:
Median Latitude: 81.364713 * Median Longitude: 23.264967 * South-bound Latitude: 77.467200 * West-bound Longitude: 10.230300 * North-bound Latitude: 83.685100 * East-bound Longitude: 33.880600
Date/Time Start: 2017-01-11T08:07:08 * Date/Time End: 2018-01-10T22:38:44
Minimum DEPTH, water: 0.0 m * Maximum DEPTH, water: 800.0 m
Event(s):
PNC17_NS1-95 * Latitude: 80.602200 * Longitude: 13.676700 * Date/Time: 2017-01-11T08:07:08 * Elevation: -200.0 m * Campaign: PNC17 * Basis: Helmer Hanssen * Method/Device: WP-2 towed closing plankton net (WP2)
PNC17_NS1-105 * Latitude: 80.635300 * Longitude: 13.852500 * Date/Time: 2017-01-11T13:08:03 * Elevation: -200.0 m * Campaign: PNC17 * Basis: Helmer Hanssen * Method/Device: Midwater trawl (MWT)
PNC17_NS4-112 * Latitude: 80.670600 * Longitude: 12.837800 * Date/Time: 2017-01-11T21:09:02 * Elevation: -1119.0 m * Campaign: PNC17 * Basis: Helmer Hanssen * Method/Device: Midwater Ring Net (MIK-N)
Comment:
For the ROV net data, Date/Time refers to the start of the ice station. The start and end of the ROV net sampling can be found in the columns "Date/Time start" and "Date/Time end".
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventKunisch, Erin H
2Species, unique identificationSpecies UIDKunisch, Erin H
3Species, unique identification (Semantic URI)Species UID (Semantic URI)Kunisch, Erin H
4Species, unique identification (URI)Species UID (URI)Kunisch, Erin H
5Sample IDSample IDKunisch, Erin H
6Station labelStationKunisch, Erin H
7DATE/TIMEDate/TimeKunisch, Erin HGeocode
8LATITUDELatitudeKunisch, Erin HGeocode
9LONGITUDELongitudeKunisch, Erin HGeocode
10DEPTH, waterDepth watermKunisch, Erin HGeocode – minimum
11DEPTH, waterDepth watermKunisch, Erin HGeocode – maximum
12Depth commentDepth commKunisch, Erin H
13Depth, bathymetricBathy depthmKunisch, Erin H
14GearGearKunisch, Erin H
15NameNameKunisch, Erin HExport
16Date/time startDate/time startKunisch, Erin H
17Date/time endDate/time endKunisch, Erin H
18Sample amountN#Kunisch, Erin H
19Sample, dry massSample dmmgKunisch, Erin HAnalytical balance, KERN, ABT 500-5Mfreeze dried
20Absolute lipid contentLipidmgKunisch, Erin HAnalytical balance, KERN, ABT 500-5M
21Lipids, total, per dry massLipid tot dm%Kunisch, Erin HCalculated
22Tetradecanoic acid of total fatty acids14:0%Kunisch, Erin HGas chromatograph, Agilent, 6890N
23(E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-olPhytol%Kunisch, Erin HGas chromatograph, Agilent, 6890N
24iso-Pentadecanoic acid of total fatty acids (IUPAC: 13-methyltetradecanoic acid)i-15:0 (IUPAC: 13-Me-C14:0)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
2512-methyl-Tetradecanoic acid of total fatty acids (IUPAC: 12-methyltetradecanoic acid)ai-15:0 (IUPAC: 12-Me-C14:0)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
26Pentadecanoic acid of total fatty acids15:0%Kunisch, Erin HGas chromatograph, Agilent, 6890N
27Hexadecanoic acid of total fatty acids16:0%Kunisch, Erin HGas chromatograph, Agilent, 6890N
28cis-9-Hexadecenoic acid of total fatty acids (IUPAC: (9Z)-hexadec-9-enoic acid)16:1(n-7) (IUPAC: C16:1w7c)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
29cis-11-Hexadecenoic acid of total fatty acids (IUPAC: (11Z)-hexadec-11-enoic acid)16:1(n-5) (IUPAC: C16:1w5c)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
309,12-Hexadecadienoic acid of total fatty acids16:2(n-4)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
316,9,12-Hexadecatrienoic acid of total fatty acids16:3(n-4)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
326,9,12,15-Hexadecatetraenoic acid of total fatty acids16:4(n-1)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
33Heptadecanoic acid of total fatty acids17:0%Kunisch, Erin HGas chromatograph, Agilent, 6890N
34cis-9-Heptadecenoic acid of total fatty acids17:1(n-8)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
35Octadecanoic acid of total fatty acids18:0%Kunisch, Erin HGas chromatograph, Agilent, 6890N
36cis-9-Octadecenoic acid of total fatty acids (IUPAC: Octadec-9-enoic acid)18:1(n-9) (IUPAC: C18:1w9)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
37cis-11-Octadecenoic acid of total fatty acids (IUPAC: Octadec-11-enoic acid)18:1(n-7) (IUPAC: C18:1w7)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
38cis-13-Octadecenoic acid of total fatty acids18:1(n-5)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
39all-cis-9,12-Octadecadienoic acid of total fatty acids18:2(n-6)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
40all-cis-6,9,12-Octadecatrienoic acid of total fatty acids18:3(n-6)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
41all-cis-9,12,15-Octadecatrienoic acid of total fatty acids18:3(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
42all-cis-6,9,12,15-Octadecatetraenoic acid of total fatty acids18:4(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
43Icosanoic acid of total fatty acids20:0%Kunisch, Erin HGas chromatograph, Agilent, 6890N
44cis-9-Icosanoic acid of total fatty acids20:1(n-11)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
45cis-11-Icosenoic acid of total fatty acids20:1(n-9)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
46cis-13-Icosenoic acid of total fatty acids20:1(n-7)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
47all-cis-8,11-Eicosasadienoic acid of total fatty acids20:2(n-9)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
48all-cis-11,14,17-Eicosatrienoic acid of total fatty acids20:3(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
49all-cis-5,8,11,14-Eicosatetraenoic acid of total fatty acids20:4(n-6)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
50all-cis-8,11,14,17-Eicosatetraenoic acid of total fatty acids20:4(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
51all-cis-5,8,11,14,17-Eicosapentaenoic acid of total fatty acids20:5(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
52cis-11-Docosenoic acid of total fatty acids22:1(n-11)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
53cis-13-Docosenoic acid of total fatty acids22:1(n-9)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
54cis-15-Docosenoic acid of total fatty acids22:1(n-7)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
55all-cis-7,10,13,16,19-Docosapentaenoic acid of total fatty acids22:5(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
56all-cis-4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids22:6(n-3)%Kunisch, Erin HGas chromatograph, Agilent, 6890N
57Tetradecanoic acid, δ13C14:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
58Pentadecanoic acid, δ13C15:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
59Hexadecanoic acid, δ13C16:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
60cis-11-Hexadecenoic acid, δ13C16:1(n-5) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
61cis-9-Hexadecenoic acid, δ13C16:1(n-7) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
629,12-Hexadecadienoic acid, δ13C16:2(n-4) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
636,9,12-Hexadecatrienoic acid, δ13C16:3(n-4) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
64Heptadecanoic acid, δ13C17:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
65Octadecanoic acid, δ13C18:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
66cis-13-Octadecenoic acid, δ13C18:1(n-5) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
67cis-11-Octadecenoic acid, δ13C18:1(n-7) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
68cis-9-Octadecenoic acid, δ13C18:1(n-9) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
69all-cis-9,12-Octadecadienoic acid, δ13C18:2(n-6) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
70all-cis-9,12,15-Octadecatrienoic acid, δ13C18:3(n-3) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
71all-cis-6,9,12-Octadecatrienoic acid, δ13C18:3(n-6) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
72all-cis-6,9,12,15-Octadecatetraenoic acid, δ13C18:4(n-3) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
73Icosanoic acid, δ13C20:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
74cis-9-Icosanoic acid, δ13C20:1(n-11) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
75cis-11-Icosenoic acid, δ13C20:1(n-9) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
76cis-13-Icosenoic acid, δ13C20:1(n-7) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
77all-cis-11,14-Eicosadienoic acid, δ13C20:2(n-6) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
78all-cis-11,14,17-Eicosatrienoic acid, δ13C20:3(n-3) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
79all-cis-8,11,14,17-Eicosatetraenoic acid, δ13C20:4(n-3) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
80all-cis-5,8,11,14-Eicosatetraenoic acid, δ13C20:4(n-6) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
81all-cis-5,8,11,14,17-Eicosapentaenoic acid, δ13C20:5(n-3) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
82Heneicosanoic acid, δ13C21:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
83Docosanoic acid, δ13C22:0 δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
84cis-11-Docosenoic acid, δ13C22:1(n-11) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
85cis-13-Docosenoic acid, δ13C22:1(n-9) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
86cis-15-Docosenoic acid, δ13C22:1(n-7) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
87all-cis-13,16-Docosadienoic acid, δ13C22:2(n-6) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
88all-cis-4,7,10,13,16,19-Docosahexaenoic acid, δ13C22:6(n-3) δ13C‰ PDBKunisch, Erin HGas chromatograph, Thermo Fisher Scientific, TRACE GC Ultra; coupled with Isotope ratio mass spectrometer, Thermo Fisher Scientific, Delta V Plus
89Wax estersWax esters%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
90TriacylglycerolsTAG%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
91Fatty alcoholsFatty alc%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
92SterolsSterol%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
93Fatty acids, freeFFA%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
94PhosphatidylethanolaminePE%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
95PhosphatidylinositolPI%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
96PhosphatidylserinePS%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
97PhosphatidylcholinePC%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
98Lyso-PhosphatidylcholineslysoPC%Kunisch, Erin HHigh performance liquid chromatography (HPLC) system, VWR, LaChrome Elite; coupled with evaporative light scattering detector (ELSD), Sedere, SEDEX 75Column: Chromolith Performance-Si, 100 x 4,6mm (Merck, Part: 1.514.650.001); Software: EZChrome Elite Version 3.1.7
Size:
3159 data points

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